Detailed information of TA system TA132185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2628717..2629357
• Replicon: chromosome
• Accession: NZ_CP041836
• Organism: Mycobacterium tuberculosis strain 2-0031P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FPJ72_RS12065
• Protein ID: WP_003412970.1
• Coordinates: 2628717..2629136 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FPJ72_RS12070
• Protein ID: WP_003412975.1
• Coordinates: 2629133..2629357 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ72_RS12035	2624307..2625029	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FPJ72_RS12040	2625546..2625768	+	223	Protein_2382	type II toxin-antitoxin system VapB family antitoxin	-
FPJ72_RS12045	2625765..2626166	+	402	WP_003412963.1	PIN domain-containing protein	-
FPJ72_RS12050	2626201..2627121	-	921	WP_003412965.1	restriction endonuclease	-
FPJ72_RS12055	2627462..2627707	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ72_RS12060	2627766..2628716	+	951	WP_003911916.1	Lsr2 family protein	-
FPJ72_RS12065	2628717..2629136	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ72_RS12070	2629133..2629357	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FPJ72_RS12075	2629388..2632231	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FPJ72_RS12080	2632303..2632704	-	402	WP_003412981.1	hypothetical protein	-
FPJ72_RS12085	2632704..2633174	-	471	WP_003899364.1	transcription antitermination factor NusB	-
FPJ72_RS12090	2633177..2633740	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T132185 WP_003412970.1 NZ_CP041836:c2629136-2628717 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T132185 NZ_CP041836:c2629136-2628717 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT132185 WP_003412975.1 NZ_CP041836:c2629357-2629133 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT132185 NZ_CP041836:c2629357-2629133 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09