Detailed information of TA system TA132149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3696738..3697412
• Replicon: chromosome
• Accession: NZ_CP041835
• Organism: Mycobacterium tuberculosis strain 2-0034P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: FPJ73_RS17365
• Protein ID: WP_003417282.1
• Coordinates: 3696738..3697166 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: FPJ73_RS17370
• Protein ID: WP_003417286.1
• Coordinates: 3697170..3697412 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ73_RS17340	3692560..3692961	-	402	WP_003417264.1	cytidine deaminase	-
FPJ73_RS17345	3693102..3693536	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
FPJ73_RS17350	3693533..3693967	+	435	WP_003902441.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
FPJ73_RS17355	3694096..3695868	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
FPJ73_RS17360	3695868..3696659	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
FPJ73_RS17365	3696738..3697166	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ73_RS17370	3697170..3697412	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ73_RS17375	3697534..3698148	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
FPJ73_RS17380	3698145..3698810	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
FPJ73_RS17385	3698811..3699344	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
FPJ73_RS17390	3699341..3699715	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
FPJ73_RS17395	3699812..3700876	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T132149 WP_003417282.1 NZ_CP041835:c3697166-3696738 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T132149 NZ_CP041835:c3697166-3696738 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT132149 WP_003417286.1 NZ_CP041835:c3697412-3697170 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT132149 NZ_CP041835:c3697412-3697170 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59