Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3099560..3100130 Replicon chromosome
Accession NZ_CP041835
Organism Mycobacterium tuberculosis strain 2-0034P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ73_RS14625 Protein ID WP_003414166.1
Coordinates 3099560..3099916 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ73_RS14630 Protein ID WP_003901465.1
Coordinates 3099900..3100130 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ73_RS14605 3095012..3096700 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ73_RS14610 3096704..3097030 - 327 WP_003414157.1 hypothetical protein -
FPJ73_RS14615 3097203..3097790 + 588 WP_003914429.1 DUF3558 family protein -
FPJ73_RS14620 3097809..3099458 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ73_RS14625 3099560..3099916 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ73_RS14630 3099900..3100130 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ73_RS14635 3100173..3101216 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ73_RS14640 3101407..3101682 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ73_RS14645 3101858..3102112 - 255 WP_003917684.1 hypothetical protein -
FPJ73_RS14650 3102260..3102664 + 405 WP_003414181.1 hypothetical protein -
FPJ73_RS14655 3102661..3102852 + 192 WP_003414184.1 hypothetical protein -
FPJ73_RS14660 3102916..3104205 + 1290 Protein_2893 transposase family protein -
FPJ73_RS14665 3104439..3104696 + 258 WP_003899489.1 hypothetical protein -
FPJ73_RS14670 3104801..3105112 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T132143 WP_003414166.1 NZ_CP041835:c3099916-3099560 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T132143 NZ_CP041835:c3099916-3099560 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT132143 WP_003901465.1 NZ_CP041835:c3100130-3099900 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT132143 NZ_CP041835:c3100130-3099900 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References