Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3059563..3060242 Replicon chromosome
Accession NZ_CP041835
Organism Mycobacterium tuberculosis strain 2-0034P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag FPJ73_RS14405 Protein ID WP_003414059.1
Coordinates 3059563..3059979 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ73_RS14410 Protein ID WP_003414061.1
Coordinates 3059976..3060242 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ73_RS14385 3055615..3056517 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FPJ73_RS14390 3056586..3057338 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ73_RS14395 3057582..3057857 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ73_RS14400 3057854..3059476 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
FPJ73_RS14405 3059563..3059979 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
FPJ73_RS14410 3059976..3060242 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ73_RS14415 3060268..3060663 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
FPJ73_RS14420 3060660..3060929 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ73_RS14425 3060939..3062033 - 1095 WP_003414068.1 restriction endonuclease subunit S -
FPJ73_RS14430 3062030..3062449 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ73_RS14435 3062523..3063002 - 480 WP_003414073.1 dihydrofolate reductase -
FPJ73_RS14440 3063073..3063873 - 801 WP_031713946.1 thymidylate synthase -
FPJ73_RS14445 3064029..3064766 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T132141 WP_003414059.1 NZ_CP041835:c3059979-3059563 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T132141 NZ_CP041835:c3059979-3059563 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT132141 WP_003414061.1 NZ_CP041835:c3060242-3059976 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT132141 NZ_CP041835:c3060242-3059976 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References