Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 759666..760206 Replicon chromosome
Accession NZ_CP041834
Organism Mycobacterium tuberculosis strain 2-0043-unknownP6C4

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag FPJ74_RS03480 Protein ID WP_003403376.1
Coordinates 759666..759974 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag FPJ74_RS03485 Protein ID WP_003403381.1
Coordinates 759961..760206 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ74_RS03455 754981..756486 + 1506 WP_193055467.1 carotenoid cleavage oxygenase -
FPJ74_RS03460 756567..757577 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
FPJ74_RS03465 757965..758348 - 384 WP_003403365.1 ribonuclease VapC6 -
FPJ74_RS03470 758443..758598 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ74_RS03475 758674..759390 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
FPJ74_RS03480 759666..759974 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FPJ74_RS03485 759961..760206 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
FPJ74_RS03490 760316..760753 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
FPJ74_RS03495 760750..761004 - 255 WP_003911263.1 antitoxin VapB7 -
FPJ74_RS03500 761118..763481 + 2364 WP_193055468.1 arylsulfatase AtsD -
FPJ74_RS03505 763544..763870 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ74_RS03510 763782..764120 + 339 WP_003403405.1 PIN domain-containing protein -
FPJ74_RS03515 764117..764290 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T132046 WP_003403376.1 NZ_CP041834:c759974-759666 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T132046 NZ_CP041834:c759974-759666 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT132046 WP_003403381.1 NZ_CP041834:c760206-759961 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT132046 NZ_CP041834:c760206-759961 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References