Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3122169..3122739 Replicon chromosome
Accession NZ_CP041833
Organism Mycobacterium tuberculosis strain 2-0046P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ75_RS14660 Protein ID WP_003414166.1
Coordinates 3122169..3122525 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ75_RS14665 Protein ID WP_003901465.1
Coordinates 3122509..3122739 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ75_RS14640 3117621..3119309 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ75_RS14645 3119313..3119639 - 327 WP_003414157.1 hypothetical protein -
FPJ75_RS14650 3119812..3120399 + 588 WP_003914429.1 DUF3558 family protein -
FPJ75_RS14655 3120418..3122067 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ75_RS14660 3122169..3122525 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ75_RS14665 3122509..3122739 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ75_RS14670 3122782..3123825 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ75_RS14675 3124016..3124291 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ75_RS14680 3124467..3124721 - 255 WP_003917684.1 hypothetical protein -
FPJ75_RS14685 3124869..3125273 + 405 WP_009938577.1 hypothetical protein -
FPJ75_RS14690 3125270..3125461 + 192 WP_003414184.1 hypothetical protein -
FPJ75_RS14695 3125525..3126814 + 1290 Protein_2900 transposase family protein -
FPJ75_RS14700 3127048..3127305 + 258 WP_003899489.1 hypothetical protein -
FPJ75_RS14705 3127410..3127721 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T132022 WP_003414166.1 NZ_CP041833:c3122525-3122169 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T132022 NZ_CP041833:c3122525-3122169 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT132022 WP_003901465.1 NZ_CP041833:c3122739-3122509 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT132022 NZ_CP041833:c3122739-3122509 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References