Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2883083..2883720 Replicon chromosome
Accession NZ_CP041833
Organism Mycobacterium tuberculosis strain 2-0046P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPJ75_RS13355 Protein ID WP_003413180.1
Coordinates 2883083..2883478 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPJ75_RS13360 Protein ID WP_003413183.1
Coordinates 2883475..2883720 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ75_RS13315 2879168..2879827 + 660 WP_003900846.1 LppA family lipoprotein -
FPJ75_RS13320 2879824..2880483 + 660 WP_003900847.1 hypothetical protein -
FPJ75_RS13325 2880480..2881142 + 663 WP_031712310.1 hypothetical protein -
FPJ75_RS13330 2881139..2881417 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ75_RS13335 2881510..2881923 + 414 WP_003413164.1 PIN domain nuclease -
FPJ75_RS13340 2881962..2882219 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPJ75_RS13345 2882216..2882593 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPJ75_RS13350 2882609..2882983 - 375 WP_003413177.1 hypothetical protein -
FPJ75_RS13355 2883083..2883478 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPJ75_RS13360 2883475..2883720 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPJ75_RS13365 2884131..2884550 - 420 WP_003413190.1 A24 family peptidase -
FPJ75_RS13370 2884562..2885371 - 810 WP_003413193.1 shikimate dehydrogenase -
FPJ75_RS13375 2885368..2886621 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPJ75_RS13380 2886614..2887126 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T132017 WP_003413180.1 NZ_CP041833:c2883478-2883083 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T132017 NZ_CP041833:c2883478-2883083 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT132017 WP_003413183.1 NZ_CP041833:c2883720-2883475 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT132017 NZ_CP041833:c2883720-2883475 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References