Detailed information of TA system TA131993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244957..1245525
• Replicon: chromosome
• Accession: NZ_CP041833
• Organism: Mycobacterium tuberculosis strain 2-0046P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ75_RS05880
• Protein ID: WP_003405865.1
• Coordinates: 1245151..1245525 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ75_RS05875
• Protein ID: WP_003405863.1
• Coordinates: 1244957..1245154 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ75_RS05855	1240998..1241636	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ75_RS05860	1241726..1242733	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ75_RS05865	1242750..1243733	-	984	WP_003898731.1	hypothetical protein	-
FPJ75_RS05870	1243796..1244869	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ75_RS05875	1244957..1245154	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ75_RS05880	1245151..1245525	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ75_RS05885	1245728..1246426	+	699	WP_003898733.1	hypothetical protein	-
FPJ75_RS05890	1246544..1246729	+	186	WP_003901093.1	hypothetical protein	-
FPJ75_RS05895	1246656..1246931	-	276	WP_003405867.1	hypothetical protein	-
FPJ75_RS05900	1247174..1247497	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ75_RS05905	1247512..1248222	-	711	WP_003911399.1	hypothetical protein	-
FPJ75_RS05910	1248244..1248453	+	210	WP_003911400.1	hypothetical protein	-
FPJ75_RS05915	1248405..1249112	-	708	Protein_1165	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T131993 WP_003405865.1 NZ_CP041833:1245151-1245525 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T131993 NZ_CP041833:1245151-1245525 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT131993 WP_003405863.1 NZ_CP041833:1244957-1245154 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT131993 NZ_CP041833:1244957-1245154 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07