Detailed information of TA system TA131959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3059518..3060197
• Replicon: chromosome
• Accession: NZ_CP041832
• Organism: Mycobacterium tuberculosis strain 2-0052P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ76_RS14395
• Protein ID: WP_003414059.1
• Coordinates: 3059518..3059934 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ76_RS14400
• Protein ID: WP_003414061.1
• Coordinates: 3059931..3060197 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ76_RS14375	3055570..3056472	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ76_RS14380	3056541..3057293	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ76_RS14385	3057537..3057812	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ76_RS14390	3057809..3059431	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FPJ76_RS14395	3059518..3059934	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ76_RS14400	3059931..3060197	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ76_RS14405	3060223..3060618	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ76_RS14410	3060615..3060884	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ76_RS14415	3060894..3061988	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ76_RS14420	3061985..3062404	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ76_RS14425	3062478..3062957	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ76_RS14430	3063028..3063828	-	801	WP_003911953.1	thymidylate synthase	-
FPJ76_RS14435	3063984..3064721	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T131959 WP_003414059.1 NZ_CP041832:c3059934-3059518 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T131959 NZ_CP041832:c3059934-3059518 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT131959 WP_003414061.1 NZ_CP041832:c3060197-3059931 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT131959 NZ_CP041832:c3060197-3059931 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64