Detailed information of TA system TA131919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 691942..692588
• Replicon: chromosome
• Accession: NZ_CP041832
• Organism: Mycobacterium tuberculosis strain 2-0052P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FPJ76_RS03095
• Protein ID: WP_003403137.1
• Coordinates: 691942..692355 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FPJ76_RS03100
• Protein ID: WP_003403139.1
• Coordinates: 692352..692588 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ76_RS03075	688025..689575	+	1551	WP_003905349.1	MCE family protein	-
FPJ76_RS03080	689627..690019	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FPJ76_RS03085	690016..690273	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FPJ76_RS03090	690456..691691	-	1236	WP_003403128.1	ATP-binding protein	-
FPJ76_RS03095	691942..692355	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ76_RS03100	692352..692588	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FPJ76_RS03105	692692..693198	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FPJ76_RS03110	693312..693842	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPJ76_RS03115	693826..694587	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FPJ76_RS03120	694644..694955	+	312	WP_003403164.1	hypothetical protein	-
FPJ76_RS03125	695027..695977	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
FPJ76_RS03130	696218..696802	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FPJ76_RS03135	696804..697514	+	711	Protein_621	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T131919 WP_003403137.1 NZ_CP041832:c692355-691942 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T131919 NZ_CP041832:c692355-691942 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT131919 WP_003403139.1 NZ_CP041832:c692588-692352 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT131919 NZ_CP041832:c692588-692352 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89