Detailed information of TA system TA131897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2913170..2913783
• Replicon: chromosome
• Accession: NZ_CP041831
• Organism: Mycobacterium tuberculosis strain 2-0059P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: FPJ77_RS13485
• Protein ID: WP_003407786.1
• Coordinates: 2913170..2913574 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FPJ77_RS13490
• Protein ID: WP_009935474.1
• Coordinates: 2913580..2913783 (-)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ77_RS13475	2909121..2911418	+	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-
FPJ77_RS13480	2911411..2913153	+	1743	WP_031714230.1	malto-oligosyltrehalose trehalohydrolase	-
FPJ77_RS13485	2913170..2913574	-	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FPJ77_RS13490	2913580..2913783	-	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ77_RS13495	2913836..2915125	-	1290	WP_003407781.1	threonine ammonia-lyase	-
FPJ77_RS13500	2915160..2915606	-	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FPJ77_RS13505	2915616..2916764	-	1149	Protein_2666	MMPL family transporter	-
FPJ77_RS13510	2916948..2917556	-	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
FPJ77_RS13515	2917624..2918001	-	378	WP_003407771.1	fumarate reductase subunit FrdD	-
FPJ77_RS13520	2917998..2918378	-	381	WP_003407768.1	fumarate reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T131897 WP_003407786.1 NZ_CP041831:c2913574-2913170 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T131897 NZ_CP041831:c2913574-2913170 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT131897 WP_009935474.1 NZ_CP041831:c2913783-2913580 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT131897 NZ_CP041831:c2913783-2913580 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46