Detailed information of TA system TA131894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2485551..2486254
• Replicon: chromosome
• Accession: NZ_CP041831
• Organism: Mycobacterium tuberculosis strain 2-0059P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ77_RS11610
• Protein ID: WP_003409778.1
• Coordinates: 2485925..2486254 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ77_RS11605
• Protein ID: WP_003409780.1
• Coordinates: 2485551..2485928 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ77_RS11565	2480709..2480900	+	192	WP_003409876.1	hypothetical protein	-
FPJ77_RS11570	2480921..2481823	+	903	WP_003899097.1	hypothetical protein	-
FPJ77_RS11575	2481834..2482184	+	351	WP_003899096.1	hypothetical protein	-
FPJ77_RS11580	2482298..2482480	+	183	WP_003409870.1	hypothetical protein	-
FPJ77_RS11585	2482473..2482874	-	402	WP_003409869.1	hypothetical protein	-
FPJ77_RS11590	2482938..2483390	+	453	WP_003899095.1	lipoprotein	-
FPJ77_RS11595	2483545..2484909	-	1365	WP_031716974.1	HNH endonuclease	-
FPJ77_RS11600	2484964..2485554	+	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ77_RS11605	2485551..2485928	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ77_RS11610	2485925..2486254	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ77_RS11615	2486464..2487234	-	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ77_RS11620	2487231..2488292	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ77_RS11625	2488289..2488804	-	516	WP_003409718.1	flavin reductase family protein	-
FPJ77_RS11630	2488801..2489871	-	1071	WP_003899091.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T131894 WP_003409778.1 NZ_CP041831:2485925-2486254 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T131894 NZ_CP041831:2485925-2486254 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT131894 WP_003409780.1 NZ_CP041831:2485551-2485928 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT131894 NZ_CP041831:2485551-2485928 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8