Detailed information of TA system TA131882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1818169..1818809
• Replicon: chromosome
• Accession: NZ_CP041831
• Organism: Mycobacterium tuberculosis strain 2-0059P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FPJ77_RS08455
• Protein ID: WP_003412970.1
• Coordinates: 1818390..1818809 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FPJ77_RS08450
• Protein ID: WP_003412975.1
• Coordinates: 1818169..1818393 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ77_RS08430	1813786..1814349	+	564	WP_003412989.1	elongation factor P	-
FPJ77_RS08435	1814352..1814822	+	471	WP_003899364.1	transcription antitermination factor NusB	-
FPJ77_RS08440	1814822..1815223	+	402	WP_003412981.1	hypothetical protein	-
FPJ77_RS08445	1815295..1818138	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FPJ77_RS08450	1818169..1818393	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FPJ77_RS08455	1818390..1818809	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ77_RS08460	1818810..1819760	-	951	WP_003911916.1	Lsr2 family protein	-
FPJ77_RS08465	1819819..1820064	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ77_RS08470	1820405..1821325	+	921	WP_003412965.1	restriction endonuclease	-
FPJ77_RS08475	1821360..1821761	-	402	WP_003412963.1	PIN domain-containing protein	-
FPJ77_RS08480	1821758..1821985	-	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ77_RS08485	1822502..1823224	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T131882 WP_003412970.1 NZ_CP041831:1818390-1818809 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T131882 NZ_CP041831:1818390-1818809 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT131882 WP_003412975.1 NZ_CP041831:1818169-1818393 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT131882 NZ_CP041831:1818169-1818393 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09