Detailed information of TA system TA131844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3174184..3174871
• Replicon: chromosome
• Accession: NZ_CP041830
• Organism: Mycobacterium tuberculosis strain 2-0068P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FPJ78_RS15010
• Protein ID: WP_003414624.1
• Coordinates: 3174428..3174871 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: FPJ78_RS15005
• Protein ID: WP_003414620.1
• Coordinates: 3174184..3174441 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ78_RS14985	3169504..3170358	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FPJ78_RS14990	3170414..3171577	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPJ78_RS14995	3171594..3172808	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FPJ78_RS15000	3172816..3174057	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FPJ78_RS15005	3174184..3174441	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ78_RS15010	3174428..3174871	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ78_RS15015	3174951..3175613	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FPJ78_RS15020	3175709..3175900	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ78_RS15025	3176232..3177980	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FPJ78_RS15030	3178076..3178657	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FPJ78_RS15035	3178757..3179023	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T131844 WP_003414624.1 NZ_CP041830:3174428-3174871 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T131844 NZ_CP041830:3174428-3174871 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT131844 WP_003414620.1 NZ_CP041830:3174184-3174441 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT131844 NZ_CP041830:3174184-3174441 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0