Detailed information of TA system TA131822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2193032..2193576
• Replicon: chromosome
• Accession: NZ_CP041830
• Organism: Mycobacterium tuberculosis strain 2-0068P6C4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TLU9
• Locus tag: FPJ78_RS10145
• Protein ID: WP_003409896.1
• Coordinates: 2193032..2193328 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P67299
• Locus tag: FPJ78_RS10150
• Protein ID: WP_003409899.1
• Coordinates: 2193325..2193576 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ78_RS10095	2188912..2189328	-	417	Protein_1994	hypothetical protein	-
FPJ78_RS10100	2189349..2189540	-	192	WP_003409876.1	hypothetical protein	-
FPJ78_RS10105	2189541..2189837	-	297	WP_003409877.1	hypothetical protein	-
FPJ78_RS10110	2190077..2190292	+	216	WP_003409878.1	antitoxin	-
FPJ78_RS10115	2190289..2190600	+	312	WP_003409881.1	type II toxin-antitoxin system VapC family toxin	-
FPJ78_RS10120	2190574..2191095	-	522	WP_003904745.1	hypothetical protein	-
FPJ78_RS10125	2191070..2191447	+	378	WP_010886136.1	type II toxin-antitoxin system toxin HigB	-
FPJ78_RS10130	2191489..2191938	+	450	WP_003409886.1	type II toxin-antitoxin system antitoxin HigA	-
FPJ78_RS10135	2191935..2192480	+	546	WP_003409891.1	SecB-like chaperone	-
FPJ78_RS10140	2192369..2192983	-	615	WP_003901296.1	hypothetical protein	-
FPJ78_RS10145	2193032..2193328	-	297	WP_003409896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ78_RS10150	2193325..2193576	-	252	WP_003409899.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FPJ78_RS10155	2193563..2194057	+	495	WP_003899099.1	hypothetical protein	-
FPJ78_RS10160	2194217..2194624	-	408	WP_003409913.1	type II toxin-antitoxin system VapC family toxin	-
FPJ78_RS10165	2194628..2194900	-	273	WP_003899100.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ78_RS10170	2194933..2196153	-	1221	WP_003409919.1	TetR family transcriptional regulator Mce3R	-
FPJ78_RS10175	2197052..2197849	+	798	WP_003899101.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.64 Da
• Isoelectric Point: 6.8604

>T131822 WP_003409896.1 NZ_CP041830:c2193328-2193032 [Mycobacterium tuberculosis]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL

Nucleotide

• Length: 297 bp

>T131822 NZ_CP041830:c2193328-2193032 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9207.17 Da
• Isoelectric Point: 7.0150

>AT131822 WP_003409899.1 NZ_CP041830:c2193576-2193325 [Mycobacterium tuberculosis]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR

Nucleotide

• Length: 252 bp

>AT131822 NZ_CP041830:c2193576-2193325 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TLU9

Antitoxin

• AlphaFold DB: A0A7U4BUZ2