Detailed information of TA system TA131781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3099703..3100273
• Replicon: chromosome
• Accession: NZ_CP041829
• Organism: Mycobacterium tuberculosis strain 3-0059P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ79_RS14615
• Protein ID: WP_003414166.1
• Coordinates: 3099703..3100059 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ79_RS14620
• Protein ID: WP_003901465.1
• Coordinates: 3100043..3100273 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ79_RS14595	3095155..3096843	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FPJ79_RS14600	3096847..3097173	-	327	WP_003414157.1	hypothetical protein	-
FPJ79_RS14605	3097346..3097933	+	588	WP_003914429.1	DUF3558 family protein	-
FPJ79_RS14610	3097952..3099601	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FPJ79_RS14615	3099703..3100059	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ79_RS14620	3100043..3100273	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ79_RS14625	3100316..3101359	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ79_RS14630	3101550..3101825	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
FPJ79_RS14635	3102001..3102255	-	255	WP_003917684.1	hypothetical protein	-
FPJ79_RS14640	3102403..3102807	+	405	WP_009938577.1	hypothetical protein	-
FPJ79_RS14645	3102804..3102995	+	192	WP_003414184.1	hypothetical protein	-
FPJ79_RS14650	3103059..3104348	+	1290	Protein_2891	transposase family protein	-
FPJ79_RS14655	3104582..3104839	+	258	WP_003899489.1	hypothetical protein	-
FPJ79_RS14660	3104944..3105255	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3099703..3114249	14546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T131781 WP_003414166.1 NZ_CP041829:c3100059-3099703 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T131781 NZ_CP041829:c3100059-3099703 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT131781 WP_003901465.1 NZ_CP041829:c3100273-3100043 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT131781 NZ_CP041829:c3100273-3100043 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3