Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3060119..3060806 Replicon chromosome
Accession NZ_CP041829
Organism Mycobacterium tuberculosis strain 3-0059P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag FPJ79_RS14405 Protein ID WP_003414064.1
Coordinates 3060411..3060806 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ79_RS14400 Protein ID WP_003414061.1
Coordinates 3060119..3060385 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ79_RS14375 3055758..3056660 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FPJ79_RS14380 3056729..3057481 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ79_RS14385 3057725..3058000 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ79_RS14390 3057997..3059619 - 1623 WP_003906897.1 type I restriction-modification system subunit M -
FPJ79_RS14395 3059706..3060122 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
FPJ79_RS14400 3060119..3060385 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ79_RS14405 3060411..3060806 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
FPJ79_RS14410 3060803..3061072 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ79_RS14415 3061082..3062176 - 1095 WP_003414068.1 restriction endonuclease subunit S -
FPJ79_RS14420 3062173..3062592 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ79_RS14425 3062666..3063145 - 480 WP_003414073.1 dihydrofolate reductase -
FPJ79_RS14430 3063216..3064016 - 801 WP_009939104.1 thymidylate synthase -
FPJ79_RS14435 3064172..3064909 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T131780 WP_003414064.1 NZ_CP041829:c3060806-3060411 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T131780 NZ_CP041829:c3060806-3060411 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT131780 WP_003414061.1 NZ_CP041829:c3060385-3060119 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT131780 NZ_CP041829:c3060385-3060119 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References