Detailed information of TA system TA131776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2860065..2860702
• Replicon: chromosome
• Accession: NZ_CP041829
• Organism: Mycobacterium tuberculosis strain 3-0059P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ79_RS13305
• Protein ID: WP_003413180.1
• Coordinates: 2860065..2860460 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPJ79_RS13310
• Protein ID: WP_003413183.1
• Coordinates: 2860457..2860702 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ79_RS13265	2855468..2856679	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ79_RS13270	2856806..2857465	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ79_RS13275	2857462..2858124	+	663	WP_003900848.1	hypothetical protein	-
FPJ79_RS13280	2858121..2858399	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ79_RS13285	2858492..2858905	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ79_RS13290	2858944..2859201	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ79_RS13295	2859198..2859575	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ79_RS13300	2859591..2859965	-	375	WP_003413177.1	hypothetical protein	-
FPJ79_RS13305	2860065..2860460	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ79_RS13310	2860457..2860702	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ79_RS13315	2861113..2861532	-	420	WP_003413190.1	A24 family peptidase	-
FPJ79_RS13320	2861544..2862353	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ79_RS13325	2862350..2863603	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ79_RS13330	2863596..2864108	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T131776 WP_003413180.1 NZ_CP041829:c2860460-2860065 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T131776 NZ_CP041829:c2860460-2860065 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT131776 WP_003413183.1 NZ_CP041829:c2860702-2860457 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT131776 NZ_CP041829:c2860702-2860457 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31