Detailed information of TA system TA131775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2858944..2859575
• Replicon: chromosome
• Accession: NZ_CP041829
• Organism: Mycobacterium tuberculosis strain 3-0059P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ79_RS13295
• Protein ID: WP_003413174.1
• Coordinates: 2859198..2859575 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ79_RS13290
• Protein ID: WP_003413167.1
• Coordinates: 2858944..2859201 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ79_RS13260	2854858..2855172	+	315	WP_009937839.1	hypothetical protein	-
FPJ79_RS13265	2855468..2856679	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ79_RS13270	2856806..2857465	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ79_RS13275	2857462..2858124	+	663	WP_003900848.1	hypothetical protein	-
FPJ79_RS13280	2858121..2858399	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ79_RS13285	2858492..2858905	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ79_RS13290	2858944..2859201	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ79_RS13295	2859198..2859575	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ79_RS13300	2859591..2859965	-	375	WP_003413177.1	hypothetical protein	-
FPJ79_RS13305	2860065..2860460	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ79_RS13310	2860457..2860702	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ79_RS13315	2861113..2861532	-	420	WP_003413190.1	A24 family peptidase	-
FPJ79_RS13320	2861544..2862353	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ79_RS13325	2862350..2863603	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ79_RS13330	2863596..2864108	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T131775 WP_003413174.1 NZ_CP041829:2859198-2859575 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T131775 NZ_CP041829:2859198-2859575 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT131775 WP_003413167.1 NZ_CP041829:2858944-2859201 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT131775 NZ_CP041829:2858944-2859201 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCGGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5