Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3152808..3153412 Replicon chromosome
Accession NZ_CP041828
Organism Mycobacterium tuberculosis strain 3-0090P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag FPJ80_RS14830 Protein ID WP_003414492.1
Coordinates 3152808..3153200 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag FPJ80_RS14835 Protein ID WP_003414495.1
Coordinates 3153197..3153412 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ80_RS14800 3147958..3148746 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
FPJ80_RS14805 3149080..3149625 - 546 WP_003904931.1 DUF1802 family protein -
FPJ80_RS14810 3149897..3150781 - 885 WP_193042908.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
FPJ80_RS14815 3150784..3151671 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
FPJ80_RS14820 3151976..3152521 - 546 WP_003908074.1 DUF1802 family protein -
FPJ80_RS14825 3152518..3152787 - 270 WP_003414489.1 DUF2277 family protein -
FPJ80_RS14830 3152808..3153200 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
FPJ80_RS14835 3153197..3153412 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FPJ80_RS14840 3153459..3154208 + 750 WP_003908075.1 enoyl-CoA hydratase -
FPJ80_RS14845 3154287..3155369 - 1083 WP_003908076.1 ABC transporter ATP-binding protein -
FPJ80_RS14850 3155362..3156672 - 1311 WP_193042909.1 ABC transporter substrate-binding protein -
FPJ80_RS14855 3156675..3157502 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
FPJ80_RS14860 3157499..3158410 - 912 WP_003904073.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T131722 WP_003414492.1 NZ_CP041828:c3153200-3152808 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T131722 NZ_CP041828:c3153200-3152808 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT131722 WP_003414495.1 NZ_CP041828:c3153412-3153197 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT131722 NZ_CP041828:c3153412-3153197 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References