Detailed information of TA system TA131720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3123714..3124284
• Replicon: chromosome
• Accession: NZ_CP041828
• Organism: Mycobacterium tuberculosis strain 3-0090P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ80_RS14680
• Protein ID: WP_003414166.1
• Coordinates: 3123714..3124070 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ80_RS14685
• Protein ID: WP_003901465.1
• Coordinates: 3124054..3124284 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ80_RS14660	3119166..3120854	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FPJ80_RS14665	3120858..3121184	-	327	WP_003414157.1	hypothetical protein	-
FPJ80_RS14670	3121357..3121944	+	588	WP_003914429.1	DUF3558 family protein	-
FPJ80_RS14675	3121963..3123612	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FPJ80_RS14680	3123714..3124070	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ80_RS14685	3124054..3124284	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ80_RS14690	3124327..3125370	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ80_RS14695	3125561..3125836	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
FPJ80_RS14700	3126012..3126266	-	255	WP_003917684.1	hypothetical protein	-
FPJ80_RS14705	3126414..3126818	+	405	WP_003414181.1	hypothetical protein	-
FPJ80_RS14710	3126815..3127006	+	192	WP_003414184.1	hypothetical protein	-
FPJ80_RS14715	3127070..3128359	+	1290	Protein_2904	transposase family protein	-
FPJ80_RS14720	3128593..3128850	+	258	WP_003899489.1	hypothetical protein	-
FPJ80_RS14725	3128955..3129266	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3113749..3137691	23942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T131720 WP_003414166.1 NZ_CP041828:c3124070-3123714 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T131720 NZ_CP041828:c3124070-3123714 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT131720 WP_003901465.1 NZ_CP041828:c3124284-3124054 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT131720 NZ_CP041828:c3124284-3124054 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3