Detailed information of TA system TA131703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2236089..2236777
• Replicon: chromosome
• Accession: NZ_CP041828
• Organism: Mycobacterium tuberculosis strain 3-0090P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ80_RS10330
• Protein ID: WP_193042892.1
• Coordinates: 2236089..2236508 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: FPJ80_RS10335
• Protein ID: WP_003409968.1
• Coordinates: 2236517..2236777 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ80_RS10310	2231584..2232432	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
FPJ80_RS10315	2232395..2233840	-	1446	WP_003409946.1	APC family permease	-
FPJ80_RS10320	2234019..2234705	-	687	WP_193042891.1	immunoprotective protein Mpt64	-
FPJ80_RS10325	2234896..2235864	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
FPJ80_RS10330	2236089..2236508	-	420	WP_193042892.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ80_RS10335	2236517..2236777	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ80_RS10340	2236920..2238596	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
FPJ80_RS10345	2238584..2239237	-	654	WP_003409976.1	cutinase Cfp21	-
FPJ80_RS10350	2239352..2239582	+	231	WP_003409980.1	DUF167 domain-containing protein	-
FPJ80_RS10355	2239667..2240578	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FPJ80_RS10360	2240687..2241286	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14742.93 Da
• Isoelectric Point: 7.9535

>T131703 WP_193042892.1 NZ_CP041828:c2236508-2236089 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFMRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T131703 NZ_CP041828:c2236508-2236089 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTATGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT131703 WP_003409968.1 NZ_CP041828:c2236777-2236517 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT131703 NZ_CP041828:c2236777-2236517 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C483