Detailed information of TA system TA131665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3736549..3737223
• Replicon: chromosome
• Accession: NZ_CP041827
• Organism: Mycobacterium tuberculosis strain 3-0096P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: FPJ81_RS17490
• Protein ID: WP_003417282.1
• Coordinates: 3736549..3736977 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: FPJ81_RS17495
• Protein ID: WP_003417286.1
• Coordinates: 3736981..3737223 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ81_RS17465	3732371..3732772	-	402	WP_003417264.1	cytidine deaminase	-
FPJ81_RS17470	3732913..3733347	+	435	WP_072460198.1	succinate dehydrogenase, cytochrome b556 subunit	-
FPJ81_RS17475	3733344..3733778	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
FPJ81_RS17480	3733907..3735679	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
FPJ81_RS17485	3735679..3736470	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
FPJ81_RS17490	3736549..3736977	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ81_RS17495	3736981..3737223	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ81_RS17500	3737345..3737959	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
FPJ81_RS17505	3737956..3738621	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
FPJ81_RS17510	3738622..3739155	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
FPJ81_RS17515	3739152..3739526	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
FPJ81_RS17520	3739623..3740270	-	648	Protein_3460	cyclic pyranopterin phosphate synthase MoaA	-
FPJ81_RS17530	3741590..3742117	-	528	Protein_3462	radical SAM protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T131665 WP_003417282.1 NZ_CP041827:c3736977-3736549 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T131665 NZ_CP041827:c3736977-3736549 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT131665 WP_003417286.1 NZ_CP041827:c3737223-3736981 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT131665 NZ_CP041827:c3737223-3736981 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59