Detailed information of TA system TA131645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2281343..2281969
• Replicon: chromosome
• Accession: NZ_CP041827
• Organism: Mycobacterium tuberculosis strain 3-0096P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPJ81_RS10575
• Protein ID: WP_003410075.1
• Coordinates: 2281571..2281969 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPJ81_RS10570
• Protein ID: WP_003911750.1
• Coordinates: 2281343..2281570 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ81_RS10560	2279382..2279726	-	345	WP_003410065.1	ferredoxin family protein	-
FPJ81_RS10565	2279915..2281084	-	1170	WP_003899126.1	ATP-binding protein	-
FPJ81_RS10570	2281343..2281570	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ81_RS10575	2281571..2281969	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FPJ81_RS10580	2282152..2282583	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPJ81_RS10585	2282684..2283118	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPJ81_RS10590	2283555..2283734	-	180	Protein_2093	hypothetical protein	-
FPJ81_RS10595	2283759..2284985	+	1227	Protein_2094	IS110 family transposase	-
FPJ81_RS10600	2285113..2285487	-	375	Protein_2095	HNH endonuclease	-
FPJ81_RS10605	2285538..2286799	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T131645 WP_003410075.1 NZ_CP041827:2281571-2281969 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T131645 NZ_CP041827:2281571-2281969 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT131645 WP_003911750.1 NZ_CP041827:2281343-2281570 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT131645 NZ_CP041827:2281343-2281570 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8