Detailed information of TA system TA131592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2902098..2902729
• Replicon: chromosome
• Accession: NZ_CP041826
• Organism: Mycobacterium tuberculosis strain 3-0124P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ82_RS13455
• Protein ID: WP_003413174.1
• Coordinates: 2902352..2902729 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ82_RS13450
• Protein ID: WP_003413167.1
• Coordinates: 2902098..2902355 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ82_RS13420	2898012..2898326	+	315	WP_009937839.1	hypothetical protein	-
FPJ82_RS13425	2898622..2899833	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ82_RS13430	2899960..2900619	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ82_RS13435	2900616..2901278	+	663	WP_003903936.1	hypothetical protein	-
FPJ82_RS13440	2901275..2901553	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ82_RS13445	2901646..2902059	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ82_RS13450	2902098..2902355	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ82_RS13455	2902352..2902729	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ82_RS13460	2902745..2903119	-	375	WP_003413177.1	hypothetical protein	-
FPJ82_RS13465	2903219..2903614	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ82_RS13470	2903611..2903856	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ82_RS13475	2904267..2904686	-	420	WP_003413190.1	A24 family peptidase	-
FPJ82_RS13480	2904698..2905507	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ82_RS13485	2905504..2906757	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ82_RS13490	2906750..2907262	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T131592 WP_003413174.1 NZ_CP041826:2902352-2902729 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T131592 NZ_CP041826:2902352-2902729 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT131592 WP_003413167.1 NZ_CP041826:2902098-2902355 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT131592 NZ_CP041826:2902098-2902355 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5