Detailed information of TA system TA131584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2285551..2286177
• Replicon: chromosome
• Accession: NZ_CP041826
• Organism: Mycobacterium tuberculosis strain 3-0124P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPJ82_RS10585
• Protein ID: WP_003410075.1
• Coordinates: 2285779..2286177 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPJ82_RS10580
• Protein ID: WP_003911750.1
• Coordinates: 2285551..2285778 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ82_RS10570	2283590..2283934	-	345	WP_003410065.1	ferredoxin family protein	-
FPJ82_RS10575	2284123..2285292	-	1170	WP_003899126.1	ATP-binding protein	-
FPJ82_RS10580	2285551..2285778	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ82_RS10585	2285779..2286177	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FPJ82_RS10590	2286360..2286791	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPJ82_RS10595	2286892..2287326	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPJ82_RS10600	2287763..2287942	-	180	Protein_2095	hypothetical protein	-
FPJ82_RS10605	2288003..2288650	+	648	WP_071854215.1	transposase	-
FPJ82_RS10610	2288604..2289194	+	591	WP_003907840.1	IS110 family transposase	-
FPJ82_RS10615	2289322..2289696	-	375	Protein_2098	HNH endonuclease	-
FPJ82_RS10620	2289747..2291008	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T131584 WP_003410075.1 NZ_CP041826:2285779-2286177 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T131584 NZ_CP041826:2285779-2286177 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT131584 WP_003911750.1 NZ_CP041826:2285551-2285778 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT131584 NZ_CP041826:2285551-2285778 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8