Detailed information of TA system TA131539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3172067..3172635
• Replicon: chromosome
• Accession: NZ_CP041825
• Organism: Mycobacterium tuberculosis strain 4-0010P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ83_RS14835
• Protein ID: WP_003405865.1
• Coordinates: 3172067..3172441 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ83_RS14840
• Protein ID: WP_003405863.1
• Coordinates: 3172438..3172635 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ83_RS14800	3168480..3169187	+	708	Protein_2921	adenylate/guanylate cyclase domain-containing protein	-
FPJ83_RS14805	3169139..3169348	-	210	WP_003911400.1	hypothetical protein	-
FPJ83_RS14810	3169370..3170080	+	711	WP_003911399.1	hypothetical protein	-
FPJ83_RS14815	3170095..3170418	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ83_RS14820	3170661..3170936	+	276	WP_003405867.1	hypothetical protein	-
FPJ83_RS14825	3170863..3171048	-	186	WP_003901093.1	hypothetical protein	-
FPJ83_RS14830	3171166..3171864	-	699	WP_003898733.1	hypothetical protein	-
FPJ83_RS14835	3172067..3172441	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ83_RS14840	3172438..3172635	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ83_RS14845	3172723..3173796	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ83_RS14850	3173859..3174842	+	984	WP_003898731.1	hypothetical protein	-
FPJ83_RS14855	3174859..3175866	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ83_RS14860	3175956..3176594	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T131539 WP_003405865.1 NZ_CP041825:c3172441-3172067 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T131539 NZ_CP041825:c3172441-3172067 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT131539 WP_003405863.1 NZ_CP041825:c3172635-3172438 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT131539 NZ_CP041825:c3172635-3172438 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07