Detailed information of TA system TA131533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2647961..2648574
• Replicon: chromosome
• Accession: NZ_CP041825
• Organism: Mycobacterium tuberculosis strain 4-0010P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: FPJ83_RS12505
• Protein ID: WP_003407786.1
• Coordinates: 2647961..2648365 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FPJ83_RS12510
• Protein ID: WP_009935474.1
• Coordinates: 2648371..2648574 (-)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ83_RS12495	2643912..2646209	+	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-
FPJ83_RS12500	2646202..2647944	+	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
FPJ83_RS12505	2647961..2648365	-	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FPJ83_RS12510	2648371..2648574	-	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ83_RS12515	2648627..2649916	-	1290	WP_003407781.1	threonine ammonia-lyase	-
FPJ83_RS12520	2649951..2650397	-	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FPJ83_RS12525	2650407..2651555	-	1149	Protein_2471	MMPL family transporter	-
FPJ83_RS12530	2651739..2652347	-	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
FPJ83_RS12535	2652415..2652792	-	378	WP_003407771.1	fumarate reductase subunit FrdD	-
FPJ83_RS12540	2652789..2653169	-	381	WP_003407768.1	fumarate reductase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T131533 WP_003407786.1 NZ_CP041825:c2648365-2647961 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T131533 NZ_CP041825:c2648365-2647961 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT131533 WP_009935474.1 NZ_CP041825:c2648574-2648371 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT131533 NZ_CP041825:c2648574-2648371 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46