Detailed information of TA system TA131518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1558805..1559445
• Replicon: chromosome
• Accession: NZ_CP041825
• Organism: Mycobacterium tuberculosis strain 4-0010P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FPJ83_RS07475
• Protein ID: WP_003412970.1
• Coordinates: 1559026..1559445 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FPJ83_RS07470
• Protein ID: WP_003412975.1
• Coordinates: 1558805..1559029 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ83_RS07450	1554422..1554985	+	564	WP_003412989.1	elongation factor P	-
FPJ83_RS07455	1554988..1555458	+	471	WP_003899364.1	transcription antitermination factor NusB	-
FPJ83_RS07460	1555458..1555859	+	402	WP_003412981.1	hypothetical protein	-
FPJ83_RS07465	1555931..1558774	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FPJ83_RS07470	1558805..1559029	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FPJ83_RS07475	1559026..1559445	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ83_RS07480	1559446..1560396	-	951	WP_003911916.1	Lsr2 family protein	-
FPJ83_RS07485	1560455..1560700	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ83_RS07490	1561041..1561961	+	921	WP_003412965.1	restriction endonuclease	-
FPJ83_RS07495	1561996..1562397	-	402	WP_003412963.1	PIN domain-containing protein	-
FPJ83_RS07500	1562394..1562621	-	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ83_RS07505	1563138..1563860	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T131518 WP_003412970.1 NZ_CP041825:1559026-1559445 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T131518 NZ_CP041825:1559026-1559445 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT131518 WP_003412975.1 NZ_CP041825:1558805-1559029 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT131518 NZ_CP041825:1558805-1559029 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09