Detailed information of TA system TA131459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2193643..2194346
• Replicon: chromosome
• Accession: NZ_CP041824
• Organism: Mycobacterium tuberculosis strain 4-0012P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ84_RS10055
• Protein ID: WP_003409778.1
• Coordinates: 2193643..2193972 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ84_RS10060
• Protein ID: WP_003409780.1
• Coordinates: 2193969..2194346 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ84_RS10035	2190026..2191096	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FPJ84_RS10040	2191093..2191608	+	516	WP_003409718.1	flavin reductase family protein	-
FPJ84_RS10045	2191605..2192666	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ84_RS10050	2192663..2193433	+	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ84_RS10055	2193643..2193972	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ84_RS10060	2193969..2194346	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ84_RS10065	2194343..2194933	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ84_RS10070	2194988..2196352	+	1365	WP_031695344.1	HNH endonuclease	-
FPJ84_RS10075	2196507..2196959	-	453	WP_003899095.1	lipoprotein	-
FPJ84_RS10080	2197023..2197424	+	402	WP_003409869.1	hypothetical protein	-
FPJ84_RS10085	2197417..2197599	-	183	WP_003409870.1	hypothetical protein	-
FPJ84_RS10090	2197713..2198063	-	351	WP_003899096.1	hypothetical protein	-
FPJ84_RS10095	2198074..2198976	-	903	WP_003899097.1	hypothetical protein	-
FPJ84_RS10100	2198997..2199188	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T131459 WP_003409778.1 NZ_CP041824:c2193972-2193643 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T131459 NZ_CP041824:c2193972-2193643 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT131459 WP_003409780.1 NZ_CP041824:c2194346-2193969 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT131459 NZ_CP041824:c2194346-2193969 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8