Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2474945..2475558 Replicon chromosome
Accession NZ_CP041823
Organism Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag FPJ85_RS11760 Protein ID WP_003408465.1
Coordinates 2475169..2475558 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag FPJ85_RS11755 Protein ID WP_003408469.1
Coordinates 2474945..2475172 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ85_RS11735 2470837..2471547 + 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -
FPJ85_RS11740 2471537..2471956 + 420 WP_003408483.1 hypothetical protein -
FPJ85_RS11745 2471999..2473246 - 1248 WP_003408476.1 serine hydrolase -
FPJ85_RS11750 2473243..2474727 - 1485 WP_009937637.1 biotin carboxylase -
FPJ85_RS11755 2474945..2475172 + 228 WP_003408469.1 antitoxin Antitoxin
FPJ85_RS11760 2475169..2475558 + 390 WP_003408465.1 PIN domain-containing protein Toxin
FPJ85_RS11770 2476168..2476947 - 780 WP_003408460.1 IclR family transcriptional regulator -
FPJ85_RS11775 2476961..2477779 - 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
FPJ85_RS11780 2477832..2478181 - 350 Protein_2323 cupin domain-containing protein -
FPJ85_RS11785 2478181..2479011 - 831 WP_003906661.1 cyclase family protein -
FPJ85_RS11790 2479014..2479928 - 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T131411 WP_003408465.1 NZ_CP041823:2475169-2475558 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T131411 NZ_CP041823:2475169-2475558 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT131411 WP_003408469.1 NZ_CP041823:2474945-2475172 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT131411 NZ_CP041823:2474945-2475172 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References