Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2188067..2188653 Replicon chromosome
Accession NZ_CP041823
Organism Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FPJ85_RS10365 Protein ID WP_003410010.1
Coordinates 2188309..2188653 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FPJ85_RS10360 Protein ID WP_003410014.1
Coordinates 2188067..2188315 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ85_RS10340 2184050..2184817 - 768 WP_003410019.1 hemerythrin domain-containing protein -
FPJ85_RS10345 2184974..2185330 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FPJ85_RS10350 2185383..2185655 + 273 WP_003410017.1 DUF1490 family protein -
FPJ85_RS10355 2185652..2187967 + 2316 WP_003899120.1 cation transporter ATPase CptG -
FPJ85_RS10360 2188067..2188315 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FPJ85_RS10365 2188309..2188653 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FPJ85_RS10370 2188742..2189077 + 336 WP_003410009.1 dehydrogenase -
FPJ85_RS10375 2188978..2189235 - 258 WP_003410006.1 hypothetical protein -
FPJ85_RS10380 2189321..2189662 + 342 WP_003410003.1 DUF2384 domain-containing protein -
FPJ85_RS10385 2189659..2190219 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
FPJ85_RS10390 2190739..2191278 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FPJ85_RS10395 2191504..2191932 - 429 WP_003906713.1 cellulose-binding protein -
FPJ85_RS10400 2192348..2192947 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T131404 WP_003410010.1 NZ_CP041823:2188309-2188653 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T131404 NZ_CP041823:2188309-2188653 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT131404 WP_003410014.1 NZ_CP041823:2188067-2188315 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT131404 NZ_CP041823:2188067-2188315 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References