Detailed information of TA system TA131403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2164287..2164913
• Replicon: chromosome
• Accession: NZ_CP041823
• Organism: Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPJ85_RS10260
• Protein ID: WP_003410075.1
• Coordinates: 2164287..2164685 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPJ85_RS10265
• Protein ID: WP_003911750.1
• Coordinates: 2164686..2164913 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ85_RS10230	2159886..2161142	+	1257	WP_003410095.1	HNH endonuclease	-
FPJ85_RS10235	2161270..2161860	-	591	WP_003899131.1	IS110 family transposase	-
FPJ85_RS10240	2161814..2162461	-	648	WP_071854215.1	transposase	-
FPJ85_RS10245	2162522..2162701	+	180	Protein_2017	hypothetical protein	-
FPJ85_RS10250	2163138..2163572	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPJ85_RS10255	2163673..2164104	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPJ85_RS10260	2164287..2164685	-	399	WP_003410075.1	PIN domain nuclease	Toxin
FPJ85_RS10265	2164686..2164913	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ85_RS10270	2165172..2166341	+	1170	WP_003899126.1	ATP-binding protein	-
FPJ85_RS10275	2166530..2166874	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T131403 WP_003410075.1 NZ_CP041823:c2164685-2164287 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T131403 NZ_CP041823:c2164685-2164287 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT131403 WP_003911750.1 NZ_CP041823:c2164913-2164686 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT131403 NZ_CP041823:c2164913-2164686 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8