Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1344065..1344744 Replicon chromosome
Accession NZ_CP041823
Organism Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag FPJ85_RS06285 Protein ID WP_003414059.1
Coordinates 1344328..1344744 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ85_RS06280 Protein ID WP_003414061.1
Coordinates 1344065..1344331 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ85_RS06245 1339541..1340278 - 738 WP_003414079.1 dienelactone hydrolase family protein -
FPJ85_RS06250 1340434..1341234 + 801 WP_009939104.1 thymidylate synthase -
FPJ85_RS06255 1341305..1341784 + 480 WP_003414073.1 dihydrofolate reductase -
FPJ85_RS06260 1341858..1342277 + 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ85_RS06265 1342274..1343368 + 1095 WP_003414068.1 restriction endonuclease subunit S -
FPJ85_RS06270 1343378..1343647 + 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ85_RS06275 1343644..1344039 + 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
FPJ85_RS06280 1344065..1344331 + 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ85_RS06285 1344328..1344744 + 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
FPJ85_RS06290 1344831..1346453 + 1623 WP_003906897.1 type I restriction-modification system subunit M -
FPJ85_RS06295 1346450..1346725 + 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ85_RS06300 1346969..1347721 + 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ85_RS06305 1347790..1348692 + 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T131391 WP_003414059.1 NZ_CP041823:1344328-1344744 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T131391 NZ_CP041823:1344328-1344744 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT131391 WP_003414061.1 NZ_CP041823:1344065-1344331 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT131391 NZ_CP041823:1344065-1344331 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References