Detailed information of TA system TA131389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1302816..1303386
• Replicon: chromosome
• Accession: NZ_CP041823
• Organism: Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ85_RS06060
• Protein ID: WP_003414166.1
• Coordinates: 1303030..1303386 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ85_RS06055
• Protein ID: WP_003901465.1
• Coordinates: 1302816..1303046 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ85_RS06015	1297834..1298145	-	312	WP_003414190.1	hypothetical protein	-
FPJ85_RS06020	1298250..1298507	-	258	WP_003899489.1	hypothetical protein	-
FPJ85_RS06025	1298741..1300030	-	1290	Protein_1187	transposase family protein	-
FPJ85_RS06030	1300094..1300285	-	192	WP_003414184.1	hypothetical protein	-
FPJ85_RS06035	1300282..1300686	-	405	WP_009938577.1	hypothetical protein	-
FPJ85_RS06040	1300834..1301088	+	255	WP_003917684.1	hypothetical protein	-
FPJ85_RS06045	1301264..1301539	-	276	WP_003911993.1	DUF1778 domain-containing protein	-
FPJ85_RS06050	1301730..1302773	+	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ85_RS06055	1302816..1303046	+	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ85_RS06060	1303030..1303386	+	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ85_RS06065	1303488..1305137	-	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FPJ85_RS06070	1305156..1305743	-	588	WP_003914429.1	DUF3558 family protein	-
FPJ85_RS06075	1305916..1306242	+	327	WP_003414157.1	hypothetical protein	-
FPJ85_RS06080	1306246..1307934	+	1689	WP_003414155.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292177..1331278	39101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T131389 WP_003414166.1 NZ_CP041823:1303030-1303386 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T131389 NZ_CP041823:1303030-1303386 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT131389 WP_003901465.1 NZ_CP041823:1302816-1303046 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT131389 NZ_CP041823:1302816-1303046 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3