Detailed information of TA system TA131386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1235638..1236186
• Replicon: chromosome
• Accession: NZ_CP041823
• Organism: Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: FPJ85_RS05715
• Protein ID: WP_003414602.1
• Coordinates: 1235638..1235901 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: FPJ85_RS05720
• Protein ID: WP_003414599.1
• Coordinates: 1235905..1236186 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ85_RS05695	1230712..1231953	+	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FPJ85_RS05700	1231961..1233175	+	1215	WP_003899518.1	zinc metalloprotease Rip	-
FPJ85_RS05705	1233192..1234355	+	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPJ85_RS05710	1234411..1235265	+	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FPJ85_RS05715	1235638..1235901	-	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ85_RS05720	1235905..1236186	-	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
FPJ85_RS05725	1236458..1238269	+	1812	WP_003906921.1	penicillin-binding protein	-
FPJ85_RS05730	1238351..1238731	-	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
FPJ85_RS05735	1238728..1238976	-	249	WP_003913411.1	antitoxin VapB23	-
FPJ85_RS05740	1239080..1239664	+	585	WP_003899514.1	DUF1707 domain-containing protein	-
FPJ85_RS05745	1239706..1240563	+	858	WP_003899513.1	type I methionyl aminopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T131386 WP_003414602.1 NZ_CP041823:c1235901-1235638 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T131386 NZ_CP041823:c1235901-1235638 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT131386 WP_003414599.1 NZ_CP041823:c1236186-1235905 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT131386 NZ_CP041823:c1236186-1235905 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8