Detailed information of TA system TA131375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697363..698009
• Replicon: chromosome
• Accession: NZ_CP041823
• Organism: Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FPJ85_RS03120
• Protein ID: WP_003403137.1
• Coordinates: 697363..697776 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FPJ85_RS03125
• Protein ID: WP_003403139.1
• Coordinates: 697773..698009 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ85_RS03100	693446..694996	+	1551	WP_003403119.1	MCE family protein	-
FPJ85_RS03105	695048..695440	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FPJ85_RS03110	695437..695694	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FPJ85_RS03115	695877..697112	-	1236	WP_003403128.1	ATP-binding protein	-
FPJ85_RS03120	697363..697776	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ85_RS03125	697773..698009	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FPJ85_RS03130	698113..698619	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FPJ85_RS03135	698733..699263	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FPJ85_RS03140	699247..700008	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FPJ85_RS03145	700065..700376	+	312	WP_003403164.1	hypothetical protein	-
FPJ85_RS03150	700448..701398	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
FPJ85_RS03155	701639..702223	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FPJ85_RS03160	702225..702935	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T131375 WP_003403137.1 NZ_CP041823:c697776-697363 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T131375 NZ_CP041823:c697776-697363 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT131375 WP_003403139.1 NZ_CP041823:c698009-697773 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT131375 NZ_CP041823:c698009-697773 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89