131372

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 640446..641122 Replicon chromosome
Accession NZ_CP041823
Organism Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag FPJ85_RS02870 Protein ID WP_003898500.1
Coordinates 640446..640859 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag FPJ85_RS02875 Protein ID WP_003402915.1
Coordinates 640856..641122 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ85_RS02840 635791..636093 - 303 WP_003402896.1 DUF3349 domain-containing protein -
FPJ85_RS02845 636153..636431 - 279 WP_003402901.1 hypothetical protein -
FPJ85_RS02850 636428..637681 - 1254 WP_003402904.1 inorganic phosphate transporter family protein -
FPJ85_RS02855 637801..638187 - 387 WP_003402907.1 VOC family protein -
FPJ85_RS02860 638250..639134 - 885 WP_003402909.1 SDR family oxidoreductase -
FPJ85_RS02865 639230..640132 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
FPJ85_RS02870 640446..640859 - 414 WP_003898500.1 PIN domain-containing protein Toxin
FPJ85_RS02875 640856..641122 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
FPJ85_RS02880 641314..643029 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
FPJ85_RS02885 643107..644711 + 1605 WP_003402920.1 amidohydrolase -
FPJ85_RS02890 644708..645688 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T131372 WP_003898500.1 NZ_CP041823:c640859-640446 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T131372 NZ_CP041823:c640859-640446 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT131372 WP_003402915.1 NZ_CP041823:c641122-640856 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT131372 NZ_CP041823:c641122-640856 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References