Detailed information of TA system TA131333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1543292..1543929
• Replicon: chromosome
• Accession: NZ_CP041822
• Organism: Mycobacterium tuberculosis strain 4-0024P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ86_RS07375
• Protein ID: WP_003413180.1
• Coordinates: 1543534..1543929 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: FPJ86_RS07370
• Protein ID: WP_003413183.1
• Coordinates: 1543292..1543537 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ86_RS07350	1539886..1540398	+	513	WP_003413197.1	Holliday junction resolvase RuvX	-
FPJ86_RS07355	1540391..1541644	+	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ86_RS07360	1541641..1542450	+	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ86_RS07365	1542462..1542881	+	420	WP_003413190.1	A24 family peptidase	-
FPJ86_RS07370	1543292..1543537	+	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ86_RS07375	1543534..1543929	+	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ86_RS07380	1544029..1544403	+	375	WP_003413177.1	hypothetical protein	-
FPJ86_RS07385	1544419..1544796	-	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ86_RS07390	1544793..1545050	-	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ86_RS07395	1545089..1545502	-	414	WP_003413164.1	PIN domain nuclease	-
FPJ86_RS07400	1545595..1545873	-	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ86_RS07405	1545870..1546532	-	663	WP_003900848.1	hypothetical protein	-
FPJ86_RS07410	1546529..1547188	-	660	WP_003900846.1	LppA family lipoprotein	-
FPJ86_RS07415	1547315..1548526	-	1212	WP_003900845.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T131333 WP_003413180.1 NZ_CP041822:1543534-1543929 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T131333 NZ_CP041822:1543534-1543929 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT131333 WP_003413183.1 NZ_CP041822:1543292-1543537 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT131333 NZ_CP041822:1543292-1543537 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31