Detailed information of TA system TA131328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1302763..1303333
• Replicon: chromosome
• Accession: NZ_CP041822
• Organism: Mycobacterium tuberculosis strain 4-0024P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FPJ86_RS06065
• Protein ID: WP_003414166.1
• Coordinates: 1302977..1303333 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FPJ86_RS06060
• Protein ID: WP_003901465.1
• Coordinates: 1302763..1302993 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ86_RS06020	1297782..1298093	-	312	WP_003414190.1	hypothetical protein	-
FPJ86_RS06025	1298198..1298455	-	258	WP_003899489.1	hypothetical protein	-
FPJ86_RS06030	1298689..1299978	-	1290	Protein_1188	transposase family protein	-
FPJ86_RS06035	1300042..1300233	-	192	WP_003414184.1	hypothetical protein	-
FPJ86_RS06040	1300230..1300634	-	405	WP_009938577.1	hypothetical protein	-
FPJ86_RS06045	1300782..1301036	+	255	WP_003917684.1	hypothetical protein	-
FPJ86_RS06050	1301220..1301486	-	267	Protein_1192	DUF1778 domain-containing protein	-
FPJ86_RS06055	1301677..1302720	+	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FPJ86_RS06060	1302763..1302993	+	231	WP_003901465.1	antitoxin MazE	Antitoxin
FPJ86_RS06065	1302977..1303333	+	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FPJ86_RS06070	1303435..1305084	-	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FPJ86_RS06075	1305103..1305690	-	588	WP_003914429.1	DUF3558 family protein	-
FPJ86_RS06080	1305863..1306189	+	327	WP_003414157.1	hypothetical protein	-
FPJ86_RS06085	1306193..1307881	+	1689	WP_003414155.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292125..1331223	39098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T131328 WP_003414166.1 NZ_CP041822:1302977-1303333 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T131328 NZ_CP041822:1302977-1303333 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT131328 WP_003901465.1 NZ_CP041822:1302763-1302993 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT131328 NZ_CP041822:1302763-1302993 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3