Detailed information of TA system TA131273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1765615..1766228
• Replicon: chromosome
• Accession: NZ_CP041821
• Organism: Mycobacterium tuberculosis strain 4-0062P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: FPJ88_RS08170
• Protein ID: WP_003407786.1
• Coordinates: 1765824..1766228 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FPJ88_RS08165
• Protein ID: WP_009935474.1
• Coordinates: 1765615..1765818 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ88_RS08135	1761020..1761400	+	381	WP_003407768.1	fumarate reductase subunit C	-
FPJ88_RS08140	1761397..1761774	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
FPJ88_RS08145	1761842..1762450	+	609	WP_003902101.1	TetR/AcrR family transcriptional regulator	-
FPJ88_RS08150	1762634..1763782	+	1149	Protein_1607	MMPL family transporter	-
FPJ88_RS08155	1763792..1764238	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FPJ88_RS08160	1764273..1765562	+	1290	WP_003407781.1	threonine ammonia-lyase	-
FPJ88_RS08165	1765615..1765818	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ88_RS08170	1765824..1766228	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FPJ88_RS08175	1766245..1767987	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
FPJ88_RS08180	1767980..1770277	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T131273 WP_003407786.1 NZ_CP041821:1765824-1766228 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T131273 NZ_CP041821:1765824-1766228 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT131273 WP_009935474.1 NZ_CP041821:1765615-1765818 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT131273 NZ_CP041821:1765615-1765818 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46