Detailed information of TA system TA131230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2880129..2880766
• Replicon: chromosome
• Accession: NZ_CP041820
• Organism: Mycobacterium tuberculosis strain 4-0073P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: FPJ89_RS13370
• Protein ID: WP_003413180.1
• Coordinates: 2880129..2880524 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPJ89_RS13375
• Protein ID: WP_192991471.1
• Coordinates: 2880521..2880766 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ89_RS13330	2875532..2876743	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ89_RS13335	2876870..2877529	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ89_RS13340	2877526..2878188	+	663	WP_003900848.1	hypothetical protein	-
FPJ89_RS13345	2878185..2878463	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ89_RS13350	2878556..2878969	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ89_RS13355	2879008..2879265	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
FPJ89_RS13360	2879262..2879639	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ89_RS13365	2879655..2880029	-	375	WP_003413177.1	hypothetical protein	-
FPJ89_RS13370	2880129..2880524	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
FPJ89_RS13375	2880521..2880766	-	246	WP_192991471.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
FPJ89_RS13380	2881177..2881596	-	420	WP_003413190.1	A24 family peptidase	-
FPJ89_RS13385	2881608..2882417	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ89_RS13390	2882414..2883667	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ89_RS13395	2883660..2884172	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T131230 WP_003413180.1 NZ_CP041820:c2880524-2880129 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T131230 NZ_CP041820:c2880524-2880129 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9114.33 Da
• Isoelectric Point: 5.6007

>AT131230 WP_192991471.1 NZ_CP041820:c2880766-2880521 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALSVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT131230 NZ_CP041820:c2880766-2880521 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTTCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF