Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3098407..3098977 Replicon chromosome
Accession NZ_CP041819
Organism Mycobacterium tuberculosis strain 4-0077P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ90_RS14620 Protein ID WP_003414166.1
Coordinates 3098407..3098763 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ90_RS14625 Protein ID WP_003901465.1
Coordinates 3098747..3098977 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ90_RS14600 3093859..3095547 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ90_RS14605 3095551..3095877 - 327 WP_003414157.1 hypothetical protein -
FPJ90_RS14610 3096050..3096637 + 588 WP_003914429.1 DUF3558 family protein -
FPJ90_RS14615 3096656..3098305 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ90_RS14620 3098407..3098763 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ90_RS14625 3098747..3098977 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ90_RS14630 3099020..3100063 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ90_RS14635 3100254..3100529 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ90_RS14640 3100705..3100959 - 255 WP_003917684.1 hypothetical protein -
FPJ90_RS14645 3101107..3101511 + 405 WP_003414181.1 hypothetical protein -
FPJ90_RS14650 3101508..3101699 + 192 WP_003414184.1 hypothetical protein -
FPJ90_RS14655 3101763..3103052 + 1290 Protein_2892 transposase family protein -
FPJ90_RS14660 3103286..3103543 + 258 WP_003899489.1 hypothetical protein -
FPJ90_RS14665 3103648..3103959 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T131174 WP_003414166.1 NZ_CP041819:c3098763-3098407 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T131174 NZ_CP041819:c3098763-3098407 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT131174 WP_003901465.1 NZ_CP041819:c3098977-3098747 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT131174 NZ_CP041819:c3098977-3098747 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References