Detailed information of TA system TA131150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1687360..1687976
• Replicon: chromosome
• Accession: NZ_CP041819
• Organism: Mycobacterium tuberculosis strain 4-0077P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TJ74
• Locus tag: FPJ90_RS07840
• Protein ID: WP_003407593.1
• Coordinates: 1687659..1687976 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TJ73
• Locus tag: FPJ90_RS07835
• Protein ID: WP_003900349.1
• Coordinates: 1687360..1687662 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ90_RS07825	1683246..1685093	+	1848	WP_003407585.1	methylmalonyl-CoA mutase small subunit	-
FPJ90_RS07830	1685094..1687346	+	2253	WP_003407587.1	methylmalonyl-CoA mutase	-
FPJ90_RS07835	1687360..1687662	+	303	WP_003900349.1	type II toxin-antitoxin system antitoxin MazE4	Antitoxin
FPJ90_RS07840	1687659..1687976	+	318	WP_003407593.1	type II toxin-antitoxin system toxin endoribonuclease MazF4	Toxin
FPJ90_RS07845	1687973..1688977	+	1005	WP_003407596.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-
FPJ90_RS07850	1689030..1690319	+	1290	WP_003902090.1	serine hydrolase	-
FPJ90_RS07855	1690392..1691165	-	774	WP_003912632.1	class I SAM-dependent methyltransferase	-
FPJ90_RS07860	1691223..1691435	-	213	WP_003898900.1	dodecin family protein	-
FPJ90_RS07865	1691445..1691669	-	225	WP_160522526.1	NUDIX domain-containing protein	-
FPJ90_RS07870	1691939..1692967	+	1029	WP_003407612.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11377.97 Da
• Isoelectric Point: 7.9950

>T131150 WP_003407593.1 NZ_CP041819:1687659-1687976 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Nucleotide

• Length: 318 bp

>T131150 NZ_CP041819:1687659-1687976 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11173.42 Da
• Isoelectric Point: 9.5564

>AT131150 WP_003900349.1 NZ_CP041819:1687360-1687662 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Nucleotide

• Length: 303 bp

>AT131150 NZ_CP041819:1687360-1687662 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• PDB: 5XE3
• PDB: 5XE2

Antitoxin

• AlphaFold DB: G0TJ73