Detailed information of TA system TA131138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 753849..754641
• Replicon: chromosome
• Accession: NZ_CP041819
• Organism: Mycobacterium tuberculosis strain 4-0077P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ90_RS03465
• Protein ID: WP_003403386.1
• Coordinates: 754204..754641 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ90_RS03460
• Protein ID: WP_003403381.1
• Coordinates: 753849..754094 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ90_RS03430	748869..750374	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ90_RS03435	750455..751465	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPJ90_RS03440	751853..752236	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ90_RS03445	752331..752486	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ90_RS03450	752562..753278	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPJ90_RS03455	753554..753862	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ90_RS03460	753849..754094	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ90_RS03465	754204..754641	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ90_RS03470	754638..754892	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ90_RS03475	755006..757369	+	2364	WP_031651291.1	arylsulfatase AtsD	-
FPJ90_RS03480	757432..757758	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ90_RS03485	757670..758008	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ90_RS03490	758005..758178	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T131138 WP_003403386.1 NZ_CP041819:c754641-754204 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T131138 NZ_CP041819:c754641-754204 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT131138 WP_003403381.1 NZ_CP041819:c754094-753849 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT131138 NZ_CP041819:c754094-753849 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0