Detailed information of TA system TA131125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 361839..362410
• Replicon: chromosome
• Accession: NZ_CP041819
• Organism: Mycobacterium tuberculosis strain 4-0077P6C4

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FPJ90_RS01575
• Protein ID: WP_003401560.1
• Coordinates: 361839..362141 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FPJ90_RS01580
• Protein ID: WP_003401563.1
• Coordinates: 362189..362410 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ90_RS01555	357512..358315	-	804	WP_003401540.1	hypothetical protein	-
FPJ90_RS01560	358325..359722	-	1398	WP_003401544.1	sulfatase	-
FPJ90_RS01565	359901..361472	+	1572	WP_031651392.1	PE family protein	-
FPJ90_RS01570	361615..361842	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FPJ90_RS01575	361839..362141	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FPJ90_RS01580	362189..362410	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FPJ90_RS01585	362407..362832	+	426	WP_003401566.1	PIN domain nuclease	-
FPJ90_RS01590	362968..363600	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FPJ90_RS01595	363597..364505	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T131125 WP_003401560.1 NZ_CP041819:361839-362141 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T131125 NZ_CP041819:361839-362141 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT131125 WP_003401563.1 NZ_CP041819:362189-362410 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT131125 NZ_CP041819:362189-362410 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48