Detailed information of TA system TA131123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 73309..73942
• Replicon: chromosome
• Accession: NZ_CP041819
• Organism: Mycobacterium tuberculosis strain 4-0077P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ90_RS00370
• Protein ID: WP_003902592.1
• Coordinates: 73541..73942 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P0CW29
• Locus tag: FPJ90_RS00365
• Protein ID: WP_003400577.1
• Coordinates: 73309..73548 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ90_RS00350	68605..70065	+	1461	WP_003902591.1	FAD-binding oxidoreductase	-
FPJ90_RS00355	70101..70262	+	162	WP_003899796.1	hypothetical protein	-
FPJ90_RS00360	70303..73242	+	2940	WP_003901785.1	UPF0182 family protein	-
FPJ90_RS00365	73309..73548	+	240	WP_003400577.1	antitoxin VapB1	Antitoxin
FPJ90_RS00370	73541..73942	+	402	WP_003902592.1	type II toxin-antitoxin system ribonuclease VapC1	Toxin
FPJ90_RS00375	73994..76231	-	2238	WP_003899797.1	NADP-dependent isocitrate dehydrogenase	-
FPJ90_RS00380	76349..76918	-	570	WP_003400591.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
FPJ90_RS00385	77021..77932	+	912	WP_003899798.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (24-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14349.48 Da
• Isoelectric Point: 4.8887

>T131123 WP_003902592.1 NZ_CP041819:73541-73942 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKEPISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T131123 NZ_CP041819:73541-73942 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGCCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8717.84 Da
• Isoelectric Point: 8.5960

>AT131123 WP_003400577.1 NZ_CP041819:73309-73548 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT131123 NZ_CP041819:73309-73548 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHM7