Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2876507..2877144 Replicon chromosome
Accession NZ_CP041818
Organism Mycobacterium tuberculosis strain 4-0087P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPJ91_RS13340 Protein ID WP_003413180.1
Coordinates 2876507..2876902 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPJ91_RS13345 Protein ID WP_003413183.1
Coordinates 2876899..2877144 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ91_RS13300 2872592..2873251 + 660 WP_003900846.1 LppA family lipoprotein -
FPJ91_RS13305 2873248..2873907 + 660 WP_003900847.1 hypothetical protein -
FPJ91_RS13310 2873904..2874566 + 663 WP_003900848.1 hypothetical protein -
FPJ91_RS13315 2874563..2874841 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ91_RS13320 2874934..2875347 + 414 WP_003413164.1 PIN domain nuclease -
FPJ91_RS13325 2875386..2875643 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPJ91_RS13330 2875640..2876017 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPJ91_RS13335 2876033..2876407 - 375 WP_003413177.1 hypothetical protein -
FPJ91_RS13340 2876507..2876902 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPJ91_RS13345 2876899..2877144 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPJ91_RS13350 2877555..2877974 - 420 WP_003413190.1 A24 family peptidase -
FPJ91_RS13355 2877986..2878795 - 810 WP_003413193.1 shikimate dehydrogenase -
FPJ91_RS13360 2878792..2880045 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FPJ91_RS13365 2880038..2880550 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T131108 WP_003413180.1 NZ_CP041818:c2876902-2876507 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T131108 NZ_CP041818:c2876902-2876507 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT131108 WP_003413183.1 NZ_CP041818:c2877144-2876899 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT131108 NZ_CP041818:c2877144-2876899 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References