Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1236467..1237098 Replicon chromosome
Accession NZ_CP041818
Organism Mycobacterium tuberculosis strain 4-0087P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag FPJ91_RS05845 Protein ID WP_003898728.1
Coordinates 1236467..1236778 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag FPJ91_RS05850 Protein ID WP_003405836.1
Coordinates 1236778..1237098 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ91_RS05825 1231948..1233372 - 1425 WP_003405805.1 class II fumarate hydratase -
FPJ91_RS05830 1233403..1234491 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
FPJ91_RS05835 1234547..1235191 + 645 WP_003915494.1 DUF4245 domain-containing protein -
FPJ91_RS05840 1235198..1236355 - 1158 WP_003898727.1 AI-2E family transporter -
FPJ91_RS05845 1236467..1236778 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
FPJ91_RS05850 1236778..1237098 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
FPJ91_RS05855 1237108..1238633 + 1526 Protein_1153 carboxylesterase/lipase family protein -
FPJ91_RS05860 1238651..1239763 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
FPJ91_RS05865 1239773..1240030 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
FPJ91_RS05870 1240020..1241267 - 1248 WP_003900275.1 exodeoxyribonuclease VII large subunit -
FPJ91_RS05875 1241264..1241902 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T131083 WP_003898728.1 NZ_CP041818:c1236778-1236467 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T131083 NZ_CP041818:c1236778-1236467 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT131083 WP_003405836.1 NZ_CP041818:c1237098-1236778 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT131083 NZ_CP041818:c1237098-1236778 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References