Detailed information of TA system TA131047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2863156..2863787
• Replicon: chromosome
• Accession: NZ_CP041817
• Organism: Mycobacterium tuberculosis strain 4-0096P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ92_RS13305
• Protein ID: WP_003413174.1
• Coordinates: 2863410..2863787 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ92_RS13300
• Protein ID: WP_003413167.1
• Coordinates: 2863156..2863413 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ92_RS13270	2859071..2859385	+	315	WP_009937839.1	hypothetical protein	-
FPJ92_RS13275	2859681..2860892	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ92_RS13280	2861019..2861678	+	660	WP_003902296.1	hypothetical protein	-
FPJ92_RS13285	2861675..2862337	+	663	WP_003905878.1	hypothetical protein	-
FPJ92_RS13290	2862334..2862611	+	278	Protein_2623	type II toxin-antitoxin system VapB family antitoxin	-
FPJ92_RS13295	2862704..2863117	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ92_RS13300	2863156..2863413	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ92_RS13305	2863410..2863787	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ92_RS13310	2863803..2864177	-	375	WP_003413177.1	hypothetical protein	-
FPJ92_RS13315	2864277..2864672	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ92_RS13320	2864669..2864914	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ92_RS13325	2865325..2865744	-	420	WP_003413190.1	A24 family peptidase	-
FPJ92_RS13330	2865756..2866565	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ92_RS13335	2866562..2867815	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ92_RS13340	2867808..2868320	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T131047 WP_003413174.1 NZ_CP041817:2863410-2863787 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T131047 NZ_CP041817:2863410-2863787 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT131047 WP_003413167.1 NZ_CP041817:2863156-2863413 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT131047 NZ_CP041817:2863156-2863413 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5